rabbit polyclonal anti cd206 antibody  (Proteintech)

Journal: PLOS One

Article Title: TNFAIP3 alleviates cerebral ischemia-reperfusion injury by inhibiting M1 microglia polarization via deubiquitination of RACK1

doi: 10.1371/journal.pone.0337601

Figure Lengend Snippet: (A) Quantitative analysis of iNOS protein levels; (B) Quantitative analysis of CD16/32 protein levels; (C) Quantitative analysis of Arg-1 protein levels; (D) Quantitative analysis of CD206 protein levels. (E-F) Flow cytometry (FCM) analysis of the proportion of CD16/32 + cells in the different experimental groups; (G-H) FCM analysis of the proportion of CD206 + cells in the different experimental groups. Data are presented as the mean ± SD from replicate experiments (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. The endpoints of the line segment indicate the comparison between the two groups under consideration.

Article Snippet: Subsequently, cells were incubated with 3% BSA for 30 min at 37°C to block non-specific binding, and then exposed to primary antibodies at 4°C overnight, including anti-CD206 rabbit polyclonal antibody (1:100; cat. no. DF4149; Affinity Biosciences) and anti-CD16/32 (1:500; cat. no. ab223200; Abcam).

Techniques: Flow Cytometry, Comparison

Journal: PLOS One

Article Title: TNFAIP3 alleviates cerebral ischemia-reperfusion injury by inhibiting M1 microglia polarization via deubiquitination of RACK1

doi: 10.1371/journal.pone.0337601

Figure Lengend Snippet: (A-B) Representative images of double immunostaining and quantitative analysis of BV2 microglial M1 polarization, with CD16/32 (red) and DAPI (green); Scale bar = 100 μm. (C-D) Representative images of double immunostaining and quantitative analysis of BV2 microglial M1 polarization, with CD206 (red) and DAPI (green); Scale bar = 100 μm. Data are presented as the mean ± SD from replicate experiments (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. The endpoints of the line segment indicate the comparison between the two groups under consideration.

Article Snippet: Subsequently, cells were incubated with 3% BSA for 30 min at 37°C to block non-specific binding, and then exposed to primary antibodies at 4°C overnight, including anti-CD206 rabbit polyclonal antibody (1:100; cat. no. DF4149; Affinity Biosciences) and anti-CD16/32 (1:500; cat. no. ab223200; Abcam).

Techniques: Double Immunostaining, Comparison

Journal: PLOS One

Article Title: TNFAIP3 alleviates cerebral ischemia-reperfusion injury by inhibiting M1 microglia polarization via deubiquitination of RACK1

doi: 10.1371/journal.pone.0337601

Figure Lengend Snippet: (A) Quantitative analysis of iNOS protein levels; (B) Quantitative analysis of CD16/32 protein levels; (C) Quantitative analysis of Arg-1 protein levels; (D) Quantitative analysis of CD206 protein levels. (E-F) Flow cytometry (FCM) analysis of the proportion of CD16/32 + cells in the different experimental groups; (G-H) FCM analysis of the proportion of CD206 + cells in the different experimental groups. Data are presented as the mean ± SD from replicate experiments (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. The endpoints of the line segment indicate the comparison between the two groups under consideration.

Article Snippet: Subsequently, cells were incubated with 3% BSA for 30 min at 37°C to block non-specific binding, and then exposed to primary antibodies at 4°C overnight, including anti-CD206 rabbit polyclonal antibody (1:100; cat. no. DF4149; Affinity Biosciences) and anti-CD16/32 (1:500; cat. no. ab223200; Abcam).

Techniques: Flow Cytometry, Comparison

Journal: PLOS One

Article Title: TNFAIP3 alleviates cerebral ischemia-reperfusion injury by inhibiting M1 microglia polarization via deubiquitination of RACK1

doi: 10.1371/journal.pone.0337601

Figure Lengend Snippet: (A-B) Representative images of double immunostaining and quantitative analysis of BV2 microglial M1 polarization, with CD16/32 (red) and DAPI (green); Scale bar = 100 μm. (C-D) Representative images of double immunostaining and quantitative analysis of BV2 microglial M1 polarization, with CD206 (red) and DAPI (green); Scale bar = 100 μm. Data are presented as the mean ± SD from replicate experiments (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. The endpoints of the line segment indicate the comparison between the two groups under consideration.

Article Snippet: Subsequently, cells were incubated with 3% BSA for 30 min at 37°C to block non-specific binding, and then exposed to primary antibodies at 4°C overnight, including anti-CD206 rabbit polyclonal antibody (1:100; cat. no. DF4149; Affinity Biosciences) and anti-CD16/32 (1:500; cat. no. ab223200; Abcam).

Techniques: Double Immunostaining, Comparison

Journal: PLOS One

Article Title: TNFAIP3 alleviates cerebral ischemia-reperfusion injury by inhibiting M1 microglia polarization via deubiquitination of RACK1

doi: 10.1371/journal.pone.0337601

Figure Lengend Snippet: (A) Quantitative analysis of iNOS protein levels; (B) Quantitative analysis of CD16/32 protein levels; (C) Quantitative analysis of Arg-1 protein levels; (D) Quantitative analysis of CD206 protein levels. (E-F) Flow cytometry (FCM) analysis of the proportion of CD16/32 + cells in the different experimental groups; (G-H) FCM analysis of the proportion of CD206 + cells in the different experimental groups. Data are presented as the mean ± SD from replicate experiments (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. The endpoints of the line segment indicate the comparison between the two groups under consideration.

Article Snippet: Subsequently, cells were incubated with 3% BSA for 30 min at 37°C to block non-specific binding, and then exposed to primary antibodies at 4°C overnight, including anti-CD206 rabbit polyclonal antibody (1:100; cat. no. DF4149; Affinity Biosciences) and anti-CD16/32 (1:500; cat. no. ab223200; Abcam).

Techniques: Flow Cytometry, Comparison

Journal: Scientific reports

Article Title: CD206 and dust particles are prognostic biomarkers of progressive fibrosing interstitial lung disease associated with air pollutant exposure.

doi: 10.1038/s41598-025-93221-z

Figure Lengend Snippet: Fig. 3. Intrapulmonary M2 macrophage marker expression in interstitial lung disease (ILD) patients. Positive immunoreactivity to (A) CXCL13, (B) transforming growth factor β1 (TGF-β1), (C) connective tissue growth factor (CTGF), and (D) CD206 was visualized by immunohistochemistry and 3,3′-diaminobenzidine (DAB) detection chromogen (brown). Sections were counterstained with Mayer’s hematoxylin (blue). All images were taken at 400× magnification. Positivity (%) of (E) CXCL13, (F) TGF-β1, (G) CTGF, and (H) CD206 pulmonary expression was compared between ILD patients with progressive fibrosing (PF-ILD) and non-progressive fibrosing phenotype (non-PF-ILD). Results are expressed as median with interquartile range. *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: Rabbit polyclonal antibody against CD206 (catalogue No. abx177447) was obtained from Abbexa Ltd. (Cambridge, UK).

Techniques: Marker, Expressing, Immunohistochemistry

Journal: Scientific reports

Article Title: CD206 and dust particles are prognostic biomarkers of progressive fibrosing interstitial lung disease associated with air pollutant exposure.

doi: 10.1038/s41598-025-93221-z

Figure Lengend Snippet: Fig. 5. The effect of high daily average PM10 concentration on the pulmonary expression of M1/M2 macrophage markers and the amount of dust particles (DP) in the lungs of patients with interstitial lung disease. Intrapulmonary expression of (A) CXCL13, (B) interleukin 1β (IL-1β), (C) tumor necrosis factor α (TNFα), (D) fibroblast growth factor 2 (FGF-2), (E) transforming growth factor β1 (TGF-β1), (F) connective tissue growth factor (CTGF), (G) CD206, and the numbers of (H) DP-aw (dust particles in the alveolar wall cells), (I) DP-desq (dust particles in the desquamated epithelial cells) were compared between ILD patients who lived for ≥ 15 days (n = 57) and ILD patients who resided for < 15 days (n = 40) in places with daily ambient PM10 level of more than 50 µg/m3. Results are expressed as median with interquartile range. *p < 0.05; ***p < 0.001; ns not significant.

Article Snippet: Rabbit polyclonal antibody against CD206 (catalogue No. abx177447) was obtained from Abbexa Ltd. (Cambridge, UK).

Techniques: Concentration Assay, Expressing

Journal: Scientific reports

Article Title: CD206 and dust particles are prognostic biomarkers of progressive fibrosing interstitial lung disease associated with air pollutant exposure.

doi: 10.1038/s41598-025-93221-z

Figure Lengend Snippet: Fig. 7. Area under the receiver operating characteristics curve (AUROC) of the biomarkers to predict progressive fibrosing interstitial lung disease. (A) NLR (neutrophil-to-lymphocyte ratio), PLR (platelet-to- lymphocyte ratio), CXCL13 (%); (B) CTGF (connective tissue growth factor, %), IL-1β (interleukin 1β), TGF-β1 (transforming growth factor β1, %); (C) CD206 (%), DP-desq (dust particles in the desquamated epithelial cells, n), DP-aw (dust particles in the alveolar wall cells, n); (D) combined model constructed as a result of the regression equation.

Article Snippet: Rabbit polyclonal antibody against CD206 (catalogue No. abx177447) was obtained from Abbexa Ltd. (Cambridge, UK).

Techniques: Construct